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probe 3 plex negative control bio techne  (Bio-Techne corporation)


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    Structured Review

    Bio-Techne corporation probe 3 plex negative control bio techne
    Probe 3 Plex Negative Control Bio Techne, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/probe+3+plex+negative+control+bio+techne/pm37917586-264-90-94
    Average 86 stars, based on 1 article reviews
    probe 3 plex negative control bio techne - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Positive Control:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720

    Negative Control:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720

    Real-time Polymerase Chain Reaction:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720

    Recombinant:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720

    Plasmid Preparation:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720

    Software:

    Article Title: Single-cell transcriptomics identifies a WNT7A-FZD5 signaling axis that maintains fallopian tube stem cells in patient-derived organoids.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl L-Cysteine Sigma-Aldrich Cat# A9165-5G Forskolin (FSK) Bio-Techne Cat# 1099/10 A83.01 StemCell Technologies Cat# 72022 Y-27632 (ROCK inhibitor) Bio-Techne Cat# 1254/10 Cultrex Organoid Harvesting Solution Bio-Techne Cat# 3700-100-01 Matrigel (phenol red-free) Corning Cat# 356237 Rnase A Qiagen Cat# 19101 CHIR99021 Bio-Techne Cat# 4423/10 IWP-2 Bio-Techne Cat# 3533/10 LGK-974 Stratech Scientific Cat# S7143-SEL XAV-939 Stratech Scientific Cat# S1180-SEL-10 mg PRI-724 Abcam Cat# ab229168-5mg Surrogate Wnt ImmunoPrecise Cat# N001 IgG 2919 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A IgG 2921 (FZD5 inhibitor) In-house expressed (Sidhu Lab) N/A Valproic Acid (VPA) Bio-Techne Cat# 2815/100 DAPT Bio-Techne Cat# 2634/10 Estrogen Bio-Techne Cat# 2824/100 Progesterone Bio-Techne Cat# 2835/100 Brefeldin A (BFA) ThermoFisher Cat# 00-4506-51 Monensin ThermoFisher Cat# 00-4505-51 Lenti-X Concentrator TaKaRa Bio Cat# 631231 Polybrene Infection Reagent Sigma-Aldrich Cat# TR-1003-G RNasin Plus RNase inhibitor Promega Cat# N2611 dNTP Mix (10 mM ea) ThermoFisher Cat# 18427013 Normal Donkey Serum Sigma-Aldrich Cat# D9663-10ML VECTASHIELD anti-fade mounting medium Vector Laboratories Cat# H-1000 Critical commercial assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 Multiplex Fluorescent Detection Kit v2 Advanced Cell Diagnostics (ACD) Cat# 323110 RNeasy Plus Micro Kit Qiagen Cat# 74034 High-Capacity cDNA Reverse Transcription Kit ThermoFisher Cat# 4368814 RNAqueous-Micro Total RNA Isolation Kit ThermoFisher Cat# AM1931 SMARTer Stranded Total RNA-seq kit v2 - Pico Input TaKaRa Bioscience Cat# 634412 KAPA HIFI HotStart ReadyMix PCR kit Roche Diagnostics Cat# 07958935001 AMPure XP Beckman Coulter Cat# A63880 Deposited data Single-cell RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239582 Bulk RNA sequencing of human Fallopian tube WbS-reporter organoids This study GEO: GSE239581 Single Cell RNA sequencing of patientderived fibroblast and immune cells Hu et al. (2020)10 GEO: GSE132149 Single-cell RNA sequencing of patientderived Fallopian tube cells Hu et al. (2020)10 GEO: GSE132149 Experimental models: Cell lines Human Fallopian tube organoid lines This study (available upon request) N/A (Continued on next page) Cell Reports 42, 113354, November 28, 2023 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human Fallopian tube WbS-reporter organoid lines This study (available upon request) N/A Mouse oviduct organoid lines This study (available upon request) N/A Mouse oviduct WbS-reporter organoid lines This study (available upon request) N/A HEK293 cells ATCC Cat# CRL-1573 L-WNT3A cell line ATCC Cat# CRL-2647 HA-R-Spondin1-Fc 293T cell line AMSbio Cat# 3710-001-01 Experimental models: Organisms/strains Female mice, strain C57BL/6, aged 7–12 weeks Biomedical Sciences Facility, University of Oxford N/A Oligonucleotides Probe (3-plex human Positive Control) Bio-Techne Cat# 320861 Probe (3-plex mouse Positive Control) Bio-Techne Cat# 320881 Probe (3-plex Negative Control) Bio-Techne Cat# 320871 Probe (human WNT7A) Bio-Techne Cat# 408231 Probe (human AXIN2) Bio-Techne Cat# 400241-C2 Probe (human LGR5) Bio-Techne Cat# 311021-C3 Probe (mouse Wnt7a) Bio-Techne Cat# 401121 Probe (mouse Axin2) Bio-Techne Cat# 400331-C2 Probe (mouse Lgr5) Bio-Techne Cat# 312171-C3 Non-targeting siRNA Dharmacon Cat# D-001210-05-20 b-catenin (CTNNB1) siRNA_09 Dharmacon Cat# J-003482-09 b-catenin (CTNNB1) siRNA_10 Dharmacon Cat# J-003482-10 b-catenin (CTNNB1) siRNA_11 Dharmacon Cat# J-003482-11 b-catenin (CTNNB1) siRNA_12 Dharmacon Cat# J-003482-12 Non-Targeting (SMARTpool) siRNA Dharmacon Cat# D-001206-13-05 WNT7A siRNA Dharmacon Cat# M-008543-01-0005 FZD3 siRNA Dharmacon Cat# L-005502-00-0005 FZD5 siRNA Dharmacon Cat# L-005504-00-0005 FZD6 siRNA Dharmacon Cat# L-005505-00-0005 qPCR primer sequences see Table S5 N/A Recombinant DNA 7TGC (WNT signaling reporter plasmid) Fuerer & Nusse, 201024 Addgene Cat# 24304 pMD2.G (VSV-G envelope plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12259 psPAX2 (lentiviral packaging plasmid) Gift from Didier Trono (unpublished) Addgene Cat# 12260 M50 Super 8x TOPFlash Veeman et al., 200388 Addgene Cat# 12456 pcDNA.Control ThermoFisher Cat# 12489019 pcDNA.WNT7A Najdi et al., 201289 Addgene Cat# 35914 pcDNA.WNT7A-V5 MacDonald et al., 201490 Addgene Cat# 43816 pcDNA.WNT3A Najdi et al., 201289 Addgene Cat# 35908 pRK5-mFzd3-1D4 plasmid Yu et al., 201233 Addgene Cat# 42265 pRK5-mFzd5-1D4 plasmid Yu et al., 201233 Addgene Cat# 42267 pRK5-mFzd6-1D4 plasmid Yu et al., 201233 Addgene Cat# 42268 pCMV-hERa plasmid Mao et al., 200891 Addgene Cat# 101141 pcDNA3-PRb plasmid Su et al., 201292 Addgene Cat# 89130 Software and algorithms ZEN 3.0 image analysis software Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html (Continued on next page) 22 Cell Reports 42, 113354, November 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism v8/9 GraphPad software https://www.graphpad.com/ scientific-software/prism/ R Statistical Software (v4.1.1) R Project for Statistical Computing https://www.R-project.org/ RRID: SCR_001905 Seurat (v4.3.0) Hao et al. (2021)93 https://satijalab.org/seurat/ RRID:SCR_016341 edgeR (v3.36.0) Robinson et al. (2010)94 https://bioconductor.org/packages/ 3.14/bioc/html/edgeR.html RRID:SCR_012802 MSigDB (v2022.1.Hs) Subramanian et al. (2005)95 https://www.gsea-msigdb.org/ gsea/msigdb/index.jsp RRID:SCR_016863 fgsea (v1.20.0) Sergushichev (2016)96 https://bioconductor.org/packages/ 3.14/bioc/html/fgsea.html RRID:SCR_020938 STAR (v2.7.3a) Dobin et al. (2013)97 https://github.com/alexdobin/STAR FeatureCounts (v2.0.0) Liao et al. (2014)98 https://github.com/ShiLab- Bioinformatics/subread ToppFun Chen et al. (2009)99 https://toppgene.cchmc.org/ enrichment.jsp Other ACD HybEZTM II Hybridization System Bio-Techne Cat# 321720



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